LOCUS       Exported                3835 bp ds-DNA    circular SYN 17-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0066_sensor_prhlRRR12-sfGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Daniel Gerngross
  TITLE     Direct Submission
  JOURNAL   Exported 17 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..126
                     /note="T0"
     terminator      27..121
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    127..196
                     /note="R2oDNA spacer"
                     /note="R2oDNA 70bp spacer 29"
     terminator      203..230
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    complement(238..308)
                     /note="taR12"
     promoter        complement(309..359)
                     /note="P(Rhl) (I14017)"
     misc_feature    360..429
                     /note="spacer (B0040)"
     promoter        430..480
                     /note="P(Rhl) (I14017)"
     misc_feature    481..532
                     /note="crR12"
     RBS             515..526
                     /note="RBS (B0034)"
     CDS             533..1258
                     /codon_start=1
                     /note="sfGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRP*"
     terminator      1261..1377
                     /note="T1"
     terminator      1270..1356
                     /gene="Escherichia coli rrnB"
                     /note="T1"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1384..1403
                     /note="AB179"
     primer_bind     1569..1588
                     /note="AB5"
     rep_origin      1722..2549
                     /direction=RIGHT
                     /note="p15A"
     rep_origin      1970..1972
                     /direction=RIGHT
                     /note="P15 ori from pACYC184"
     rep_origin      complement(1973..2517)
                     /direction=LEFT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     primer_bind     2621..2640
                     /note="AB180"
     oriT            2651..2759
                     /note="incP origin of transfer"
     misc_feature    2731
                     /note="C->T just for test, no real mutation"
     CDS             complement(2915..3730)
                     /note="KamR"
ORIGIN
        1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg
       61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      121 ccaagatgtg agtcctgtta cgcccgtgtt ttccagtaga ataatagtga caatcgtttc
      181 agataagcaa ccaatatata aacgcagaaa ggcccacccg aaggtgagcc agtgtgatct
      241 agagatatat ggtagtagta agttaatttt cattaaccac cactaccaat cacctcctgg
      301 atttgggttt cgactataac aaaccatttt cttgcgtaag cctaccaatt catgcaggaa
      361 ggttctgtta agtaactgaa cccaatgtcg ttagtgacgc ttacctctta agaggtcact
      421 gacctaacat cctgcatgaa ttggtaggct tacgcaagaa aatggtttgt tatagtcgaa
      481 gaattctacc attcacctct tggatttggg tattaaagag gagaaaggta ccatgagcaa
      541 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      601 tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg gaaaactcac
      661 ccttaaattt atttgcacta ctggaaaact acctgttccg tggccaacac ttgtcactac
      721 tctgacctat ggtgttcaat gcttttcccg ttatccggat cacatgaaac ggcatgactt
      781 tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt tcaaagatga
      841 cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatcgtat
      901 cgagttaaag ggtattgatt ttaaagaaga tggaaacatt cttggacaca aactcgagta
      961 caactttaac tcacacaatg tatacatcac ggcagacaaa caaaagaatg gaatcaaagc
     1021 taacttcaaa attcgccaca acgttgaaga tggttccgtt caactagcag accattatca
     1081 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtcgac
     1141 acaatctgtc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc ttcttgagtt
     1201 tgtaactgct gctgggatta cacatggcat ggatgagctc tacaaaaggc cttaataaat
     1261 taaaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1321 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac agctgggcgc
     1381 gccaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1441 ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1501 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     1561 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     1621 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     1681 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg
     1741 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
     1801 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     1861 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1921 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1981 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     2041 cgacaggact ataaagatac caggcgtttc ccctggcggc tccctcgtgc gctctcctgt
     2101 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac
     2161 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc
     2221 ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa
     2281 gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg
     2341 aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca
     2401 agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc
     2461 aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa
     2521 gatcatctta ttaatcagat aaaatatttc tagcatgagc ggatacatat ttgaatgtat
     2581 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgctag
     2641 ggccggccta cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat
     2701 aagggacagt gaagaaggaa cacccgctcg tgggtgggcc tacttcacct atcctgcccg
     2761 gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat
     2821 atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt
     2881 atgcagcgga aaaggaccct tgtccaatta attattagaa aaattcatcc agcatcagat
     2941 gaaattgcag tttgttcata tccggattat caatgccata tttctgaaac agacgttttt
     3001 gcaggctcgg gctaaattcg cccaggcagt tccacagaat ggccagatcc tgataacgat
     3061 ccgcaatgcc cacacggccc acatcaatgc agccaatcag tttgccttca tcgaaaatca
     3121 ggttatccag gctaaaatcg ccgtgggtca ccacgctatc cgggctaaac ggcagcagtt
     3181 tatgcatttc tttccacacc tgttccaccg gccagccgtt acgttcatca tcaaaatcgc
     3241 tcgcatccac caggccgttg ttcatacggc tctgcgcctg ggccagacga aacacacgat
     3301 cgctgttaaa cgggcagttg cacaccggaa tgctatgcag acgacgcaga aacacggcca
     3361 gcgcatccac aatgttttcg ccgctatccg gatattcttc cagcacctga aacgcggttt
     3421 tgcccggaat cgcggtggtc agcagccacg catcatccgg ggtgcgaata aaatgtttaa
     3481 tggtcggcag cggcataaat tcggtcagcc agttcagacg caccatttca tcggtcacat
     3541 cgttcgccac gctgcctttg ccatgtttca gaaacagttc cggcgcatcc ggtttgccat
     3601 acagacgata aatggtcgcg ccgctctgac ccacgttatc acgcgcccat ttatagccat
     3661 acagatccgc atccatgttg ctgttcagac gcggacggct acagctcgtt tcacgctgaa
     3721 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     3781 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca aattt
//
